Alt-R CRISPR-Cas9: An optimized genome editing solution

Speaker: Dr. Abdelilah Mekhloufi, Field Application Scientist, Integrated DNA Technologies

Host: Dr. Fitsumbirhan Mehari, Technical Sales Representative, LubioScience GmbH

CRISPR: Unleashing the Potential of Genetic Engineering

CRISPR technology has been a major technological breakthrough, making genome editing easier, faster, more efficient, and more robust than previous methods.

In this seminar you will learn:

• Guidelines when designing a CRISPR experiment.

• Advantages and disadvantages of Cas9 vs Cas12 for gene editing, and their applications.

• gRNA design and formats selection recommendation

• How to optimize cell-editing with high on-target and reduced off-target activity using Alt-R HiFi CRISPR-Cas9 nuclease.

• Considerations to improve HDR (Homology Directed Repair) efficiency for knock-in experiment.

• How chemical modifications for dsDNA donor templates improve the HDR efficiency and reduce blunt insertion events.

• How and where to introduce silent mutations to improve HDR rates

• How Alt-R HDR Enhancer V2 further improves HDR rate.

Following the seminar, Food and Drinks will be served outside the seminar room. We therefore kindly ask you to register for this event.

CRISPR Lunch Seminar Bern